I was a little bit confused on how an increase in bromelain would be a bad result for the experiment. I thought bromelain would better kill the salmonella bacteria.
HI! When we first started this experiment, we believed that the more amount of Bromelain used, the better the enzyme would kill Salmonella. However that was not the case. We see this in the trend line as it continued to go up the more concentrated the dilution was which is opposite of what we thought would happen. This means that Bromelain acted as a food for the bacteria. However, we looked at our trend line and wondered if there could be a U-shaped dose response curve because of previous research showing at other groups had used extremely small doses. After making the doses smaller, Bromelain showed possible antibiotic properties.
Good job! Can you explain a bit more about what a hit dose experiment on somatic cells would entail in future studies? How would it be different than the dose response that was already done?
Hi Liana! Thank you 🙂 Using a hit dose experiment on somatic cells would confirm how Bromelain effects cells in the human body. In this experiment we were only able to see the effect it had on Salmonella Typhimurium which is a very small model organism.
Hi Delwin! Thank you for your support and good question! Walking into this class, I was interested in learning more lab techniques as well as I was hopeful to help find new antibiotics as we learned by the year 2050, more people may die from antibiotic resistance than cancer. Throughout this lab, I had amazing TAs who inspired by group to chose Bromelain as well as supported us through our research. 🙂
Do you have ideas as to why it takes such a specific dose for Bromelain to show its antibiotic properties? It’s doubtful this is due to human error because of the general u-shaped trend, correct?
Hi! Since we preformed 8 total plates, our results did not occur due to human error. I believe it took such a specific dose because of the proteolytic, anti-inflammatory, and antimicrobial properties. If we think about it, getting too much or too little of something can cause an adverse result so its important to use the dose response experiment to find the happy dilution.
Hi! Thank you for your question! I believe there are other drugs that show a u-shaped dose response curves however it was not possible for us to predict this without seeing the trendline with the 1:2 dilution.
HI! Great question. I do believe that there are other drugs that have shown this u-shaped dose response curve however it was not possible for us to predict this happening without seeing the trendline from the 1:2 dilution.
I was a little bit confused on how an increase in bromelain would be a bad result for the experiment. I thought bromelain would better kill the salmonella bacteria.
HI! When we first started this experiment, we believed that the more amount of Bromelain used, the better the enzyme would kill Salmonella. However that was not the case. We see this in the trend line as it continued to go up the more concentrated the dilution was which is opposite of what we thought would happen. This means that Bromelain acted as a food for the bacteria. However, we looked at our trend line and wondered if there could be a U-shaped dose response curve because of previous research showing at other groups had used extremely small doses. After making the doses smaller, Bromelain showed possible antibiotic properties.
Good job! Can you explain a bit more about what a hit dose experiment on somatic cells would entail in future studies? How would it be different than the dose response that was already done?
Hi Liana! Thank you 🙂 Using a hit dose experiment on somatic cells would confirm how Bromelain effects cells in the human body. In this experiment we were only able to see the effect it had on Salmonella Typhimurium which is a very small model organism.
Wow, this is some good science? Who inspired you to pursue drug discovery research?
Hi Delwin! Thank you for your support and good question! Walking into this class, I was interested in learning more lab techniques as well as I was hopeful to help find new antibiotics as we learned by the year 2050, more people may die from antibiotic resistance than cancer. Throughout this lab, I had amazing TAs who inspired by group to chose Bromelain as well as supported us through our research. 🙂
Do you have ideas as to why it takes such a specific dose for Bromelain to show its antibiotic properties? It’s doubtful this is due to human error because of the general u-shaped trend, correct?
Hi! Since we preformed 8 total plates, our results did not occur due to human error. I believe it took such a specific dose because of the proteolytic, anti-inflammatory, and antimicrobial properties. If we think about it, getting too much or too little of something can cause an adverse result so its important to use the dose response experiment to find the happy dilution.
Is a u-shaped curve for antibiotic dosing common, and therefore, was this a potentially expected result?
Hi! Thank you for your question! I believe there are other drugs that show a u-shaped dose response curves however it was not possible for us to predict this without seeing the trendline with the 1:2 dilution.
HI! Great question. I do believe that there are other drugs that have shown this u-shaped dose response curve however it was not possible for us to predict this happening without seeing the trendline from the 1:2 dilution.