10 thoughts on “G1 – Bertuccio

  1. Super interesting topic, if we did find a use for Lysin A and Lysin B in the medical field, how would you isolate it in large quantities?

    1. For medical use, we could use lytic phages with activated lysins that are known to infect the type of bacteria we want to kill. If we cannot do this with preexisting phages, I would assume this would require some genetic engineering to insert or activate lysins in the phage genome. I’m not sure we would be able to isolate lysins and have them function and survive outside of a phage, but that is definitely an intriguing idea if possible.

    1. Yes, the phamerator map is comparing the genome of Amymech to the genome of a similar phage called Giles. We have more information about Giles than we do about Amymech currently. Giles is confirmed to have a lysin A and a lysin B, so by comparing the genomes we can draw some conclusions about Amymech, because these phages are so similar. The boxes on the phamerator map are each numbered genes. The boxes shown circled in red are the lysin genes that my research was focused on.

  2. What characteristics of cluster Q phages may be attributed to also having lysin B, or how does lysin B improve the fitness of the phage

    1. Cluster Q phages are all temperate, so I suspect they may need more assistance with lysis when they switch into the lytic cycle. Lysin B simply assists lysin A, but I am not sure if the presence of lysin B necessarily improves the fitness of the phage. It would be interesting to remove or deactivate lysin B from Amymech’s genome and see how that may affect its fitness.

  3. is there anything currently known about q cluster phages that could be attributed to lysin B function? are they more infectious than other phages?

    1. Lysin B is found in 8 clusters, and 17 subclusters. I am not exactly sure how the infectivity of cluster Q phages compares to other phages, I think this would require some wet lab work. I would assume lysins are unrelated to infectivity because they are responsible for the final lysis at the end of the lytic cycle, so I don’t think they assist with the initial infection of the host.

    1. Lysins A and B can be used in phage therapy because they kill their host bacteria as they lyse it. Phage therapy works to target and kill specific bacteria, and not harm good bacteria that we want to keep. This can be super useful for fighting bacterial infections, especially with the ongoing issue of antibiotics. These lysins could be used in phage therapy alongside other lysins and antibiotics. In phage therapy, “phage cocktails” are created of several phages that could possibly infect and kill the target bacteria. This makes the phage therapy more likely to be effective.

Leave a Reply